Epigenetic and Sendromic

Sample Collection and Storage

DNA/RNA Shield™

DNA/RNA degradation in biological samples affects the accuracy of genetic identification and expression profiling studies. DNA/RNA Shield provides inactivation of nucleases and organisms containing infectious agents. It creates storage conditions at room temperature and at lower temperatures for longer storage. It does not require removal from the environment during the isolation phase.

DNA Isolation

ZymoBIOMICS™ DNA Mini Kit

ZymoBIOMICS™ DNA Microprep Kit

ZymoBIOMICS™-96 DNA Kit

ZymoBIOMICS™-96 MagBead DNA Kit

ZymoBIOMICS™ DNA/RNA Mini Kit

ZymoBIOMICS™ RNA Mini Kit

Bacterial, fungal, protozoan, algae, viral, mitochondrial and host high-quality inhibitor-free DNA is obtained from a wide range of sample sources such as feces, soil, water, biofilms, swabs, saliva, plant, food, body fluids, tissue, and cells. .

The ZymoBIOMICS™ Lysis system ensures uniform lysis of cells belonging to different organisms such as Gram-negative/positive bacteria, fungi, protozoans and algae.

ZymoBIOMICS™ DNA Mini Kit provides ideal DNA extraction for 16S rRNA gene sequencing, shotgun metagenomic sequencing, microbiome or metagenome analysis such as arrays, PCR.

Microbial Standards

ZymoBIOMICS™ Microbial Community Standard

It is a mixture of 10 inactivated microorganisms (8 bacteria and 2 fungi).

It is suitable for standardizing your DNA extraction profiles.

ZymoBIOMICS™ Microbial Community DNA Standard

ZymoBIOMICS™ Microbial Community DNA Standard is a mixture of genomic DNA obtained from 8 bacterial and 2 fungal cells.

This standard library preparation, sequencing and bioinformatics will help you evaluate errors and unexpected results that may occur in analysis.

PCR and Quantification Kits

Femto™ Fungal DNA Quantification Kit

Kit for detection and quantitation of Fungal DNA in any DNA mixture by RT-PCR.

Femto™ Bacterial DNA Quantification Kit

Detection and quantitation of Bacterial DNA in any DNA mixture by RT-PCR.

Femto™ Human DNA Quantification Kit

Kit for detection and quantitation of Human DNA in any DNA mixture by RT-PCR.

ZymoBIOMICS™ PCR Premix

It is a PCR mix containing hot-start polymerase, suitable for high sensitive applications. It can also be applied to perform other molecular downstream analyzes by adding a probe or fluorescent dye to the mix.

It performs low copy DNA amplification and 16S rRNA gene analyzes perfectly.

Microbiomics Services

For the identification of microbial compounds in any sample source with Next-Gene Sequencing;

16S rRNA ve shotgun sequencing service
Whole Genome Sequencing

You can find more details about the products in detail via the link http://www.zymoresearch.com/microbiomics, and send us your needs, requests and questions.

EPIK™ miRNA Analysis Panels

EPIK™ Cancer miRNA Hi-ROX Panel

EPIK™ Cancer miRNA Lo-ROX Panel

EPIK™ Stem Cell miRNA Hi-ROX Panel

EPIK™ Stem Cell miRNA Lo-ROX Panel

EPIK™ Biofluid miRNA Hi-ROX Panel

EPIK™ Biofluid miRNA Lo-ROX Panel

MyTaq™ Extract-PCR Kit

The MyTaq™ Extract-PCR Kit performs fast and easy DNA extraction and amplification from a variety of mammalian tissue samples (fresh, frozen, FFPE).

Conventional DNA extraction methods involve laborious steps such as phenol extraction, overnight incubation, Proteinase K treatment or column purification. Legacy methods also require large volumes of starting material and optimization is imperative for DNA extraction efficiency (especially for solid tissue samples). Biopsy samples for molecular genotyping techniques using PCR can cause problems due to the presence of bone, cartilage and blood contamination. The MyTaq Extract-PCR Kit overcomes the problems with current DNA extraction techniques and always delivers a high quality PCR product.

With the isolation buffer in the kit, DNA isolation is performed within 5-10 minutes with a column free and single tube without multi-step washing steps. Thus, DNA losses are prevented and contamination is prevented.

Suitable for fresh, frozen, FFPE tissue samples.

It provides DNA extraction from tissue sample within 10-15 minutes with a single reagent without column in a single tube.
Does not require proteinase K treatment or tissue homogenization.
The hot-start PCR mix it contains provides fast and high specific PCR with high performance.
Ideal for high-throughput genotyping from mammalian tissues, Detection of transgenes, Knockout analysis applications.

Simple Single Tube Extraction

The MyTaq Extract-PCR Kit consists of a new proteinase and a buffer system that provides efficient and rapid lysis in a single tube. The protocol requires minimal user intervention and minimizes sample loss or contamination. MyTaq Extract-PCR Kit delivers high quality PCR product in as little as 15 minutes with its simple and easy-to-use format.

High Performance PCR

The MyTaq Extract-PCR Kit is powered by the industry-leading MyTaq HS Red Mix. This product is one of Bioline's highest performance PCR mastermixes. MyTaq HS is a thermostable hot start enzyme powered by antibody-based technology. Mytaq HS increases the desired product yield by suppressing unwanted amplifications and allows set-up at room temperature. This unique optimized enzyme and buffer combination has been produced to high quality control standards and exhibits a rate, sensitivity and efficiency above other polymerases.

Direct gel loading and advanced visualization

The MyTaq Extract-PCR Kit buffer system is designed to be loaded directly onto agarose gel without the need for post-PCR processing. It improves the visualization of the red dye inside and increases efficiency in the laboratory flow.

High quality DNA generation

In experiments designed using mouse tail, MyTaq Extract-PCR Kit and its equivalent Supplier S kit were used, and the produced DNA qualities were shown. Two unique fragments of the same gene were amplified with the MyTaq Extract-PCR Kit under the same reaction conditions, yielding superior products in both cases.

DNA METHYLATION

Bisulfite Conversion

DNA Methylation Standards

DNA Methyltransferases

5-mC ELISA

5-mC Antibodies and Immunoprecipitation

Site-Specific DNA Methylation Analysis

Global 5-mC Quantification

Genome-wide 5-mC Analysis

DNA HYDROXYMETHYLATION

Hydroxymethylated DNA Standards

5-hmC Glucosyltransferase

5-hmC ELISA

5-hmC Antibodies and Enrichment

Region-Specific 5-hmC Analysis

Global 5-hmC Quantification

Genome-wide Analysis of 5-hmC

RNA METHYLATION

Bisulfite Conversation

CHROMATIN ANALYSIS

Chromatin Immunoprecipitation
Nucleosome Analysis
ChIP-Seq Services

NGS Library Preparation Kits

DNA Methylation Library Preparation Kits
DNA Hydroxymethylation Library Preparation Kits
All Genome Bisulfite Sequencing Library Kits
Reduced Representation Bisulfite Sequencing Library Kits
RNA-Seq Library Kits

Antibodies for Epigenetics

5-mC Antibody
5-hmC Antibody

Epigenetic Enzymes and Reagents

Enzymes
Nucleotides
Replacing Enzymes

High Efficiency / Automatic Isolation

96-well Turn Plate
96-well Magnetic Beads

DNA

Mytag DNA Polimerase

Mytaq DNA polymerase is a very fast and very high performance enzyme. It is designed to achieve the best results for a wide variety of templates. Thanks to its increased DNA binding feature, it provides a much faster, more sensitive and highly efficient product than other native Taq polymerase enzymes. The enzyme buffer contains dNTPs, MgCl2 and enhancers in concentrations that give the best results. It also does not require optimization. Even with the smallest amount of use, better results are obtained than other commercial Taq polymerase enzymes.The standard PCR protocol is completed within 18 minutes.

Novel buffer system does not require optimization.
Thermostable Taq enzyme is powered by antibody-based technology
Its strong stability provides the ability to dissolve and use up to 30 times.
The Red option allows direct loading of the gel without the need for loading dye.
Mastermix option is available.

Applications

Routine PCR applications
Specific amplification of complex templates
High-throughput PCR
TA cloning
Robust amplification of GC-rich sequences
Genotyping
Colony PCR
Fast PCR reactions

Fig. 1 Robust ampliication of GC-rich human genomic DNA (61% GC content)

MyTaq was compared with DNA polymerases from other suppliers for the ampliication of a 450bp fragment of the human myc gene. Decreasing amounts of human genomic DNA were used as a template (1µg, 200ng, 100ng, 50ng, 25ng and 12.5ng; lanes 1-6 respectively) in the PCR15. Marker is HyperLadder 1kb (M) (Cat No. BIO- 33025). MyTaq delivers higher yield and sensitivity as compared with all ive competing products.

Mytaq HS is an antibody based high speed and high specificity Hot Start enzyme. With its wide application, it is ideal for the most complex DNA templates. Thanks to its increased DNA binding feature, it provides a much faster, more sensitive and highly efficient product than other native Taq polymerase enzymes. The enzyme buffer contains dNTPs, MgCl2 and enhancers in concentrations that give the best results. It also does not require optimization. With its highest specificity, it suppresses non-specific product formation and primer dimer binding, while increasing the yield of the desired PCR product. It is suitable to use hot start for complex and low amount of DNA templates, multiplex PCR applications. In addition, while other commercial Taq enzymes are inhibited by bacterial cell debris or compounds in the environment and amplify only short fragments in colony PCR, MyTaq HS is tolerant to PCR inhibitors and makes amplification of long fragments without any problems.

Fastest, the standard PCR protocol is completed in 18 minutes.
It has the highest specificity.
Ability to be added to the reaction at room temperature
The novel buffer system does not require optimization.
Thermostable Taq enzyme is powered by antibody-based hot start technology
Its strong stability provides the ability to dissolve and use up to 30 times.
The Red option allows direct loading onto the gel without the need for loading dye.
Mastermix option is available.

Applications

Fast PCR reactions
Colony PCR
Genotyping
Multiplexing
Low-copy PCR assays
Low amount DNA templates
Specfiic amplification of dificult templates (GC-rich)
Assays with prolonged reaction set-up

MyFi™ is an antibody-based, new generation hot-start enzyme with a unique feature of 3.5x higher fidelity than native Taq. With high sensitivity and specificity, MyFi is the perfect choice for cloning. MyFi polymerase is supplemented by a highly optimized formulation MyFi buffer system containing specially formulated ultrapure dNTPs, MgCl and amplifiers to give the best results.

Amplification of MyFi cDNA libraries is an ideal choice for PCR amplification of targets up to 10kb long that require both high processivity and high fidelity, such as complex genomic fragments, targets with high GC content, and low copy assays. The fact that MyFi can be added to the reaction at room temperature prevents non-specific amplifications and primer-dimer formation. MyFi creates excellent PCR products with a 3'-A tail for TA cloning.

Combination of 3.5x fold fidelity and hotstart
It has the highest specificity.
The novel buffer system does not require optimization.
Thermostable Taq enzyme is powered by antibody-based hot start technology.
Its strong stability provides the ability to dissolve and use up to 30 times.
Ability to be added to the reaction at room temperature
Mastermix option is available.

Applications

Routine PCR applications
Specifically developed for TA cloning
High throughput
GC and AT-rich genomic templates
Good for low copy PCR assays
PCR requiring high specificity combined with higher fidelity

Velocity DNA Polymerase is an ultra-fast thermostable enzyme with 3´-5´ proofreading exonuclease activity, ideal for high fidelity PCR. VELOCITY offers outstanding PCR throughput even at low molds. The enzyme with very high functionality gives results in a short time for long molds. Moreover, the polymerase provides robust and reliable PCR products even in dirty or complex experiments. VELOCITY will be the best choice for your PCR applications as it covers all the polymerase functionality of an enzyme.

It has 3'-5' proofreading exonuclease activity.
It provides high processivity, high efficiency, high accuracy.
It has 50x times more fidelity than Native Taq polymerases.
Its extraordinary speed reduces reaction time.
Outstanding performance on problematic GC and AT rich targets.

Applications

Cloning techniques where high fidelity is desirable
GC-rich templates
Blunt-end cloning
Site directed mutagenesis

PCR amplification of GC-rich patterns is often hindered by the formation of hairpin-like structures and the high melting temperature. This can result in low yield of the target fragment, non-specific fragment formation, amplicons of wrong length, primer-dimer formation, and completely erroneous reactions. Although high-fidelity DNA polymerases are commonly used for routine amplification of GC-rich templates, these polymerases are still unreliable. Combined with an optimized buffer system, the unique properties of VELOCITY show superior results even with problematic molds.

Fig. 3. Amplification of GC-rich DNA fragments from human genomic DNA

VELOCITY, a competitor polymerase (P) and wild-type Pfu were compared. Lanes 1–4 are a 728bp fragment of the GP150 gene (76.9% GC), a 724bp fragment of the MRGRE gene (68% GC), a 723bp fragment of the NM_022372.3 gene (66.9% GC) and a 788bp fragment of the NM_033178.2 gene (70.9% GC) respectively. PCR was performed in 50μl reaction mixes and 5μl was run on a 1.5% TAE agarose gel. HyperLadder™ 100bp (M).

VELOCITY provides high accuracy combined with an extremely low error rate of 4.4 x 10^-7 and therefore has high functionality. These results have a fast extension rate of up to 15s/kb for plates up to 5kb and an extension rate of 30s/kb for images larger than 5kb. VELOCITY is an ideal choice for obtaining a high-throughput and mutation-free PCR product by shortening the reaction time.

DNA Clean-up and Concentration

DNA (PCR) Clean-up and Concentration
Genomic DNA Clean-up
DNA Gel Extraction
DNA Sequencing Cleaning
Size Selection
Removal of PCR Inhibitor
DNA from TRIzol and TRI Reagent

Plazmid DNA Purification

Bacterial Plasmid
Yeast Plasmid
BAC, YAC and PAC DNA

Genomic DNA

Cell and Soft Tissue DNA
Solid and FFPE Tissue DNA
Blood, Serum, Plasma and Body Fluid DNA
Serum, Plasma and Cell-Free DNA
viral DNA
Nucleosomal DNA
High Molecular Weight DNA

Microbial-Environmental DNA Isolation

DNA Markers

Enzymes

High Yield / Automated DNA Purification

RNA

RNA Cleaning

Poliacrylamide Gells
RNA Clean-up and Concentration
Removal of RT-PCR Inhibitor
RNA Gel Extraction

RNA Isolation

RNA from TRIzol and TRI Reagents
Cells ve Tissue RNA
Solid and FFPE Tissue RNA
Blood, Serum, Plasma and Body Fluid RNA
viral RNA
Bacteria, Yeast and Fungi RNA
Soil, Feces, and Plant RNA

RNA-Seq Services

RNA Ladder

DNase I

High Efficiency / Automatic Isolation

96-well Spin Plate
96-well Magnetic Beads

Sample Collection and Protection

Collection Equipments

Swab

Feces

Blood

Saliva

Urine

Tissue

Vector and Environment

Lysis Tubes

Micro RNA Expression Assay Panel

EPIK miRNA Assay provides advantages over other miRNA detection methods. miRNA-specific Reverse Transcriptase and hemi-nested Real-Time PCR primers provide extremely low background and outstanding sensitivity. Thus, it allows the precise detection of very low miRNAs and the precise discrimination of miRNA targets that are very close to each other.

*High Specificity: Only mature miRNAs are targeted, not precursors, and highly similar miRNAs are distinguished.

*Ultra Sensitivity: MiRNAs mature from at least 10 pg total RNA are detected. The RT and qPCR steps are optimized to provide high-impact amplification of low amount of input samples.

*Fast reaction: Easy two-step protocol takes less than 2 hours.

*Safe Data: 7-log linear dynamic range gives precise quantification of low and high expressed targets even with small sample amounts.

All EPIK miRNA Panel Assays are validated for the use of synthetic miRNA and human total RNA.

352 farklı kanser miRNA dublicate Array profili 176 farklı Stem Cell ve Biofluid miRNA dublicate Array profili

SensiMART qPCR mixes use DNA-Binding dye (SYBR Green), which provides better sensitivity, accurate detection, and low backround than probe-based system.

EPIK miRNA Panel Assays are powered by MIRXES technology. RT primers are designed for specific hydribization of mature miRNA targets. Following the Reverse Transcription step, newly synthesized cDNA miRNA is strongly amplified using specific forward and reverse RT-PCR primers.

The EPIK miRNA Panel Assay is optimized to use up to 100 ng of human Total RNA per 20 μl cDNA synthesis reaction. At least 10 pg of total RNA is sufficient for precise detection of highly expressed targets, while low-expressed miRNAs may require up to 100 ng of Total RNA.

EPIK miRNA Panel Assay is supplied lyophilized in 96 well plate format and is competive with most PCR instruments.

miRNA contains control DNA and primers. It provides the opportunity to work in duplicate.

All necessary reagents are included in the kit and have been formulated to minimize set-up time.

Panel Panel miRNAs

Cancer 352

Biofiluid 176

Stem Cell 176

Choose your own 4 different miRNA profiles in 4x100 reactions

If you want to work with miRNAs other than the EPIK miRNA Panel Assay panel;